|
Fig. S4
MTHs regulate WNT and NOTCH pathway genes during zebrafish embryogenesis. WISH expression of differentially expressed genes (p<0.01; FDR 5%) and comparison between control and MCT8 morphant zebrafish embryos at 48hpf. (A)WISH expression analysis of wnt1. Lateral (first panel) and dorsal (second panel) images of brain in control and MCT8 morphant zebrafish embryos at 48hpf. Red arrows denote wnt1 positive cells I the ventral hindbrain. (B) WNT1 protein secretion regulator - wls WISH expression, lateral (first panel) and dorsal (second panel) images of brain and lateral images of spinal cord in control and MCT8 morphant zebrafish embryos at 48hpf. Red arrows denote wls-expressing cells in the spinal cord. (C) Fluorescent WIHC expression analysis of DeltaA NOTCH ligand in hindbrain and spinal cord of control and MCT8 morphant embryos at 48hpf. (D) WISH expression analysis of of NOTCH pathway target gene her2 in control and MCT8 morphant zebrafish embryos at 48hpf. Lateral and dorsal images of brain (first and second panels) of analysed embryos are presented and lateral images of spinal cord are shown (lower panel). hb-hindbrain; mb-midbrain; nt-notochord In A,B,D scale bar represents 100μm. In C scale bar represents 25μm.