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Fig. 4
Growth of testis in 72?hpf embryos grafted into germ cell-depleted and castrated rag1 mutants after 2 months. (a) Histology of testes of 72?hpf embryos grafted into normal, germ cell-depleted, and castrated rag1 mutants. Dotted lines represent testes of the grafts. Scale bar, 100?µm. (b) Comparison of testis size between 2 mpf normal testes and grafts into normal, germ cell-depleted and castrated rag1 mutants. Testes volume was calculated from graft serial sections (n?=?9 normal, n?=?7 germ cell-depleted and n?=?5 castrated) and of 2 mpf males (n?=?3). Error bars indicate the standard error. *p?<?0.01; **P?<?0.05 (paired t-test).