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Fig. 2 Evaluation of mineralization of the vertebrate column in dorsomorphin-treated zebrafish. (A) The gross morphology of zebrafish aged at 7 dpf which have been treated with different concentrations of dorsomorphin (10, 20, and 30 µM, left bright-filed panel) from 3 dpf onwards. Calcein staining on control and dorsomorphin-treated embryos aged at 7 dpf (right green fluorescent panel; (B) Quantification of mineralization degree detecting the fluorescence intensity at the area of centrum form ring in the notochord. (values are mean ± SD; tested by one-way ANOVA pairwise comparison; N = fish number; Different letters indicate significant differences) (C) Chemical structure of dorsomorphin.