ZFIN Image: Shi et al., 2017, Fig. 4

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Figure Caption

Fig. 4

Rbpr2 ^musc97 mutants show eye and systemic phenotypes consistent with retinoid deficiency. Lateral view of representative WT (panels A,C) and rbpr2 homozygous mutant (rbpr2 ^musc97 mut, panels C,D) zebrafish at 5.5 dpf. Rbpr2 ^musc97 mutants showed gross defects, which included: *hydrocephaly; **smaller eyes, ***pericardial edema and ****slight tail curvature. Scale bars = 0.326 mm (A,B) and 0.103 mm (C,D). (E) qPCR analysis of rbpr2 mRNA expression from WT and rbpr2 ^musc97 mutant zebrafish larvae at 5.5 dpf. (F) Injection of WT zebrafish rbpr2 mRNA or dose specific treatment with all-trans retinoic acid (ATRA) rescues the rbpr2 ^musc97 mutant phenotype. Images obtained at 5 dpf. Rescue experiments of rbpr2 ^musc97 mutants with either mRNA or ATRA were repeated twice as outlined in methods. (G,H) Transverse sections of 5.5 dpf WT (panel G) and rbpr2 ^musc97 mutant (panel H) eyes. rbpr2 ^musc97 homozygous mutant eyes were smaller and show disruption of retinal lamination layers.

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Acknowledgments

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