IMAGE

Fig. 7

ID
ZDB-IMAGE-180417-34
Source
Figures for Rogers et al., 2017
Image
Figure Caption

Fig. 7

Expression of lefty2 responds sensitively to Nodal signaling perturbations.

Wild type embryos at the one-cell stage were injected with 0.5 pg gfp mRNA (first row, (A–B’)), 0.5 pg lft1 mRNA (second row, (C–D’)), 7.5 pg gfp mRNA (third row, (E–F’)), or 7.5 pg CA-smad2 mRNA (fourth row, (G–H’)). Embryos were fixed at 50% epiboly and shield stages and expression of endogenous lft2 (A,B,C,D,E,F,G,H) and the mesoderm marker noto (A’,B’C’,D’,E’,F’,G’H’) were assessed using in situ hybridization. Note the dramatic decrease and increase in lft2 expression after lft1 (A,B,C,D) and CA-smad2 injection (E,G,F,H), respectively. Expression of noto was relatively unchanged in all treatments.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife