ZFIN Image: Nagashima et al., 2017, Fig. 5

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Figure Caption

Fig. 5

N-cadherin is associated with actomyosin mechanical forces in differentiating cones in the pre-column zone and with M�ller glia inter-column bands in the mature mosaic. (A-A?) Flat-mount of retinal margin, immunolabeled for ZO1 (magenta) and N-Cadherin (green). (B-B??) Higher magnification of pre-column zone (boxes in A-A?). N-Cadherin labeling surrounds round cone profiles and is concentrated in adherens junction puncta (arrows) outside photoreceptor profiles. Immature rods are outlined by ZO-1 (B?, B?, yellow dots) but are negative for N-cadherin (B?). Large round profiles represent immature UV cones (B?, white dot). (C-C?) Flat-mount of retinal margin, immunolabeled for phospho-Myosin light chain II (magenta) and pan-Cadherin (green). (D-D?) Higher magnification of pre-column zone (yellow box); strong pMyoII and pan-Cadherin staining of round profiles of differentiating cones. (E-E?) Higher magnification of mature lattice mosaic (white box); pMyoII and pan-Cadherin co-localize to puncta between cone columns (arrows). Scale bar: 10 ?m (A? and C?)

Acknowledgments

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