ZFIN Image: Sawamiphak et al., 2017, Fig. 2

Image

Figure Caption

Fig. 2

Cardiomyocyte proliferation does not mediate FATC reporter expression. a Schematic illustration of the ubb:NATC construct and Cre recombination products. b Schematic illustration of the cellular events that could occur in the experiment shown in c. c NTR-mCherry⁺ (red) cardiomyocytes (H2B-GFP⁺, green) were observed in Tg(ubb:NATC);Tg(myl7:creER);Tg(myl7:H2B-GFP) larvae following MTZ-mediated cell ablation, 7 days after transient CreER activation by 4-OHT treatment at 24 hpf. All images show the same heart. d NTR-mCherry expression does not succeed cardiomyocyte DNA synthesis. The 6 h EdU pulse-labeled cells undergoing DNA synthesis (green) in 54 hpf Tg(ubb:NATC);Tg(myl7:creER) embryos. Two hours prior to EdU removal, Cre activity was induced by an 18 h 4-OHT treatment. mCerulean (blue) and NTR-mCherry (red) expression was visualized by immunostaining. No EdU⁺NTR-mCherry⁺ cardiomyocytes were detectable in 20 hearts imaged. c, d Maximum intensity projections of 40–60 μm thick confocal stacks. Representative images from a total of 12 (c) and 20 (d) animals are shown. Scale bars: 10 μm (c), 20 μm (d)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.