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Fig. 5
Expression of eGFP reporter gene in slow and fast muscle fibers of Tg(mustn1b:eGFP)iim01 embryos and larvae. eGFP expression was analyzed in the mustn1b:eGFP; Myomesin?3?RFP double transgenic fish embryos at 2 and 6 dpf. A?C: mustn1b:eGFP expression co?localized with Myomesin?3?RFP?positive slow muscles at 2 dpf. In the enlarged pictures (a?c), eGFP protein showed a sarcomeric localization in the myofibers. D?F: mustn1b:eGFP expression in Myomesin?3?RFP?positive slow muscles at 6 dpf. G,H; Localization of eGFP expression in fast muscle fibers of transgenic Zebrafish at 2 and 6 dpf, respectively. Scale bars A, D, G?=?30??m. Scale bars a?=?6??m.