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Fig. 3
Cellular morphology and VE-cadherin tensile changes occur during artery maturation. a Junctional morphology of ECs in the dorsal aorta (DA) (Venus, grey) at 2?dpf (top), 3?dpf (middle) and 4?dpf (bottom). Scale bar?=?10??m. b Junctional linearity index over time at 2?dpf (n?=?37 junctions from n?=?4 embryos), 3?dpf (n?=?37 junctions from n?=?5 embryos) and 4?dpf (n?=?51 junctions from n?=?4). Error bars represent mean?±?s.d.; 2?3?dpf **p?=?0.0010; 3?4?dpf ****p?<?0.0001, from unpaired two-sided Mann?Whitney test. c Junctional angle relative to the direction of blood flow over time at 2?dpf (n?=?53 junctions from n?=?4 embryos), 3?dpf (n?=?89 junctions from n?=?5 embryos) and 4?dpf (n?=?42 junctions from n?=?4). Error bars represent mean?±?s.d.; 2?3?dpf **p?=?0.0010; 3dpf? 4dpf ****p?<?0.0001 from unpaired two-sided Mann?Whitney test. d Heatmap image of ratio-metric FRET values in junctions at 2?dpf (top), 3?dpf (middle) and 4?dpf (bottom). Colors range from blue (?=?low FRET index/high tension) to red (?=?high FRET index/low tension). Scale bar?=?5??m. e Quantification of ratio-metric FRET values in junctions at 2?dpf (n?=?54 junctional ROIs from n?=?4 embryos), 3?dpf (n?=?79 junctional ROIs from n?=?5 embryos) and 4?dpf (n?=?71 junctional ROIs from n?=?4 embryos). Error bars represent mean?±?s.d.; 2?3?dpf ****p?<?0.0001, from unpaired two-sided t test; 3?4?dpf ****p?<?0.0001 from unpaired two-sided Mann?Whitney test. f Quantification of Teal lifetime values (nano-sec) comparing n?=?43 junctional ROIs segmented from 2?dpf embryos (n?=?8) and n?=?54 junctional ROIs from 4?dpf embryos (n?=?10). Error bars represent mean?±?s.d.; ****p?<?0.0001 from unpaired two-sided Mann?Whitney test. g Heatmap image of Teal lifetime values in junctions of 2?dpf (left) versus 4?dpf (right) embryos. Colors range from blue (?=?low lifetime/low tension) to red (?=?high lifetime/high tension). Scale bar?=?5??m