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Fig. 6 Confocal image of immunohistochemistry with Ki67 in a whole 6 dpi (fli:GFP) Casper zebrafish embryo. There were 200 to 400 CRMM-1 td-Tomato CM cells injected into the duct of Cuvier. We see tumor cell (red) migration outside the vessels (green); cell proliferation is indicated by Ki67 staining (blue). This image of the tail of a live embryo was acquired by confocal microscope (×20 dry objective). Similar images were obtained from all three CM cell lines in >10 independent experiments.
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Full text @ Invest. Ophthalmol. Vis. Sci.