Fig. 4
BRLF1 accelerates the process of mitosis in live zebrafish embryos. (A) Schematic of embryo confocal imaging. (B) Western blot analysis for BRLF1 protein levels from wild-type, injected with mRNA encoding vector-mCherry or mCherry-BRLF1 embryo after 24 h injection. (C) Time-lapse images were extracted from videos of the uninjected, injected with mRNA encoding vector-mCherry or mCherry-BRLF1 embryos. (D) Division time of the uninjected, injected with mRNA encoding vector-mCherry or mCherry-BRLF1 embryo was calculated from nuclear envelope breakdown to the formation of two daughter cells in minutes. Data are presented as means ± SD. **, P < 0.01, compared to mock.