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Fig. 2
Establishment of GBM xenografts in flk:eGFP Casper zebrafish. (A,B) 200?500 GBM tumor cells (U87-RFP, U251-RFP) were intracranial implanted into 3dpf flk:eGFP Casper zebrafish and monitored by confocal microscope every 24?hours, 293T-RFP cells implantation was set as control. Areas in dotted boxes were magnified right, arrows indicating infiltrating tumor cells. (B) The survivorship curve of U87 and U251 tumor-bearing zebrafish and control 293?T injected zebrafish. (C) A high-resolution image of intracranially implanted 293t-RFP cells at 6dpf, arrows indicating the cell debris. (D) A reconstructed 3D image of a U87-GFP xenograft in the deep brain of flk:mCherry zebrafish at 6dpf, arrows indicating infiltrating tumor cells. Z-stack serial images show the depth of the GBM xenograft in zebrafish brain, arrows indicating infiltrating tumor cells.