ZFIN Image: Herbert et al., 2017, Fig. 5

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Figure Caption

Fig. 5

Transient expression of actr10 in oligodendrocytes partially suppresses myelination defects in actr10^nl15/nl15 mutants. (A?B??) Confocal images of mbp-labeled oligodendrocytes in uninjected (A) and sox10:mRFP-actr10 (?sox10 injected?) actr10^nl15/nl15 mutants (B). While uninjected actr10^nl15/nl15 mutants do not exhibit RFP fluorescence (A? and A??), injection of sox10:mRFP-actr10 results in monomeric RFP fluorescence in actr10^nl15/nl15 mutants (B? and B??). (C?E) TEM images show dorsal spinal cords of uninjected WT and actr10^nl15/+ controls (C), uninjected actr10^nl15/nl15 mutants (D), and sox10:mRFP-actr10?injected actr10^nl15/nl15 mutants (E). Myelinated axons are pseudocolored in purple. (F) Quantification shows that sox10-injected actr10^nl15/nl15 mutants (n = 6) have significantly greater numbers of myelinated axons in the dorsal spinal cord compared with uninjected actr10^nl15/nl15 mutant siblings (n = 5, P < 0.0377), although sox10:mRFP-actr10 injection does not restore myelination to WT control levels (n = 5, P < 0.0001), indicative of partial rescue. One-way ANOVA with Tukey?s multiple comparisons test was used for statistical analyses.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA