Fig. 5

Fig. 5

Pax7⁺ cells differentiate in specific somite regions. Single confocal planes of 4 dpf wholemount immunofluorescence in lateral view. Scale bars 50 µm. A. pax7a:GFP;pfe/pfe larva showing GFP in a MyHC⁺ muscle fibre (white arrowheads). A deep GFP⁺MyHC^- cell co-labels with Myogenin (red arrowheads). B-D. Pax7 and Myogenin in epaxial somite of wt larva showing Pax7⁺Myog⁺ cells (red arrowheads) in CP (B), DE (C) and VM (D), Pax7⁺Myog^- cells (yellow arrowheads) in DE (C) and Pax7^-Myog⁺ cells (white arrowheads) in CP (B,D). Note the reduced Pax7 and Myog signal in Pax7⁺Myog⁺ cells. E-G. Time course and location of Myog⁺Pax7⁺ (E), Myog⁺Pax7^- (F) and total Myog⁺ (G) cells.