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Fig. s2 Specificity of custom-generated anti-RLTGR antibody with RL-TGR protein. (a) Confocal image of the untransfected Human Embryonic Kidney (HEK) 293 cells immunostained with anti-RL-TGR antibody (green) followed by staining with DAPI that stains nuclei blue. (b) Confocal image of HEK293 cells transfected with mammalian expression vector, pcDNA3.1 (+) containing coding region of rltgr and then immunostained with anti-RL-TGR antibody (green) followed by staining with DAPI (blue). The green fluorescence observed in cells expressing recombinant RL-TGR protein indicates that the custom generated antibodies recognize specifically the RL-TGR protein.