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Fig. 5 Inhibition of JNK signaling suppresses nuclear movement and p-Smad2 nuclear translocation in LMCs. (A-C) Transverse sections of WT, SP600125-treated and EMD 420123-treated embryos; cell membrane and nuclei were visualized by β-cat and DAPI staining, respectively at 4.7 hpf. (D,E) DNB and DCN in LMCs were measured in WT and inhibitor-treated embryos. ***P<0.001. (F) Transverse sections of WT and SP600125-treated embryos; p-Smad2 and sox32 were visualized by immunohistochemical staining and FISH, respectively. The number of embryos examined is shown bottom right. Arrowheads indicate nuclei in LMCs. Dashed lines indicate the boundary between the blastoderm and the YSL. Scale bars: 10 µm.