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Fig. 2
OTG overexpression causes dorsoanteriorized phenotypes in zebrafish embryos. (A) Un-injected control embryo. (B–D) Phenotypes of OTG mRNA-injected embryos were classified into three classes (see text). (E) Frequencies of phenotypes caused by injection of indicated amounts of OTG mRNA. (F–O) Views from animal pole, dorsal to the right (F–I,L,M). Lateral view (J,K,N,O). Expression patterns of shield markers chd (F, 20/20, 100%; G, 13/15, 87%) and gsc (H, 15/15, 100%; I, 14/15, 93%), a ventral marker bmp2 (J, 12/12, 100%; K, 11/12, 92%), a ventral mesoderm marker eve1 (L, 10/10, 100%; M, 11/12, 92%), and anterior neural marker otx2 and a posterior neural marker hoxa1 (N, 12/12, 100%; O, 15/16, 94%) in un-injected control embryos (F,H,J,L,N) or OTG mRNA-injected embryos (G,I,K,M,O) at indicated developmental stages. (P,Q) Dorsal views, anterior to the left. Expression pattern of pax6 in un-injected control (P, 20/20, 100%) or OTG mRNA-injected (Q, 14/16, 87%) embryo at 20-somite stage (22 hpf). Abbreviations. e, eye; f, forebrain; h, hindbrain; m, midbrain.