Fig. 5

Fig. 5

Excess Nodal signaling prolongs Ca²⁺ transient duration specifically in the DFCs. (A) Representative images of a single Ca²⁺ transient in a DFC at 6.5 hpf. (B) Quantification of Ca²⁺ transient duration in WT and Cyc/Ndr2-misexpressing embryos. Error bars represent standard deviation. ****, P≤0.0001. N=3 embryos in control and Cyc/Ndr2-misexpressing embryos, respectively. (C) Still images of Ca²⁺ transients in the DFCs of uninjected control and Cyc/Ndr2-misexpressing embryo in a time-lapse series. Arrowhead points to the DFC region that was imaged. (D) Quantification of DFC Ca²⁺ transient duration between DFCs and EVL cells in cyc/ndr2 RNA-injected embryos after CBX treatment. ****, P≤0.0001. N=2 embryos. (E) Quantification of DFC Ca²⁺ transient duration in Cyc/Ndr2-misexpressing embryos before and after CBX treatment. ns, not significant; N=4 embryos.