IMAGE

Fig. 1

ID
ZDB-IMAGE-180308-19
Source
Figures for Chen et al., 2017
Image
Figure Caption

Fig. 1

GCaMP6s expression and fluorescencein Tg[βactin2:GCaMP6s]stl351/stl351 and Tg[ubi:GCaMP6s]stl352/stl352 transgenic zebrafish during early embryogenesis. (A) Schematics of the Tol2[βactin2:GCaMP6s] or Tol2[ubi:GCaMP6s] constructs. (B) GCaMP6s fluorescent confocal microscope images in Tg[βactin2:GCaMP6s]stl351/stl351 and Tg[ubi:GCaMP6s]stl352/stl352 embryos at several developmental stages. a, a’, d, d’, lateral view; b, b’, c, c’, animal pole view. Asterisks indicate the yolk, and arrowheads point to the blastodisc in a, a’. Arrows point to the heart in d, d’. (C-D)RT-PCR and qRT-PCR analyses of GCaMP6s RNA expression levels in Tg[βactin2:GCaMP6s]stl351/stl351 and Tg[ubi:GCaMP6s]stl352/stl352 embryos in the course of embryogenesis. The qRT-PCR results were normalized to β-actin. Error bars represent standard deviation; N=3.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 430(2), Chen, J., Xia, L., Bruchas, M.R., Solnica-Krezel, L., Imaging early embryonic calcium activity with GCaMP6s transgenic zebrafish, 385-396, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.