Fig. 6

EHEC ingestion causes dose-dependent and strain-specific mortality in zebrafish. Zebrafish at 4 dpf were infected with 10⁸ CFU/ml (A and B) or 10⁹ CFU/ml (C and D) of EHEC (Sakai wt, blue full squares; Sakai tolA, empty squares; TUV 93-0 wt, red filled circles; TUV adhE, red empty circles) or left uninfected (black line below tolA data) and incubated at 32°C for up to 6 dpi. Fish were assessed for vital signs (presence of movement or heartbeat or circulation) every 24 h, and percent survival was plotted using Kaplan-Meier analysis (n = 15 fish per condition, over three independent experiments). Statistical significance was assessed using a Mantel-Cox test. Images of fish infected with EHEC wild-type Sakai::mCherry were taken at 21 hpi (as indicated by an arrow in each survival graph).