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Fig. 5S1
Additional telencephalon phenotypes in fezf2 mutant embryos.
(A–I) Overlay analysis of in situ expression patterns in wild-type and fezf2 mutant embryos at 2 and 3 dpf. Wild-type expression patterns are shown in green and fezf2 mutants are shown in magenta. All 3D reconstructions are generated by averaging eight or more embryos per experimental group. The position and orientation of each 2D slice within the embryo is indicated on the Nissl-stained two photon reference image to the right of the panel. Scale bar: 100 μm. (A) the fezf2 expression domain is highly disorganized in multiple regions of the diencephalon and telencephalon. (B) Vmat2 expression is largely unaffected in DA clusters of the telencephalon (arrow). (C) Expression of glyt1, a marker of astrocytic and glycinergic neuronal differentiation, is upregulated in the ventricular zone of the dorsal telencephalon (arrow). (D) Telencephalic expression of the GABAergic marker gad1b undergoes a shift away from the midline in mutant embryos (asterisk) but is not significantly reduced. (E) Expression of tbr1b, a transcription factor that plays an essential role in specifying glutamatergic pyramidal neurons, is significantly reduced. (F) Expression of emx3, a transcription factor that is broadly expressed within the telencephalon, is significantly reduced. (G) Expression of the neural progenitor marker eomesa is substantially reduced. (H) The neural progenitor marker neurog1 undergoes an anterior shift in regions of the diencephalon that closely neighbor the telencephalon (arrow). (I) The neural progenitor marker zic2a is shifted ventrally and possibly expanded in the dorsal telencephalon and surrounding regions.