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Fig. 4 Specificity of hcn4 MO. Retention of intron 3 with injection of hcn4 splice-blocking morpholino. RNA from uninjected and morphant embryos was analyzed by RT-PCR. Primers designed to amplify the exon3-exon4 boundary produced the expected PCR product in uninjected embryos (lane 2). The PCR product is too large to amplify in morphants due to inclusion of intron 3 (lane 3). To prove inclusion of intron 3 in morphant hcn4 mRNA, primers were designed to amplify the exon3-intron3 and intron3-exon4 boundaries. Products of the expected size were found in morphants (lanes 4 and 5, respectively).
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