ZFIN Image: Joris et al., 2017, Fig. S10

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Figure Caption

Fig. S10

Sequential injection of RNA and sMOsrsf5a did not prevent partial rescue to occur. Based on developmental defect severity, sMOsrsf5a injected embryos were divided into three main classes; mild, intermediate and strong. Graphs representing the proportions of the three types of embryos after the indicated treatments. The total numbers of embryos pooled from n = 3 independent experiments are indicated above each bar. The graph shows mean+SEM, a Chi-square test with two degrees of freedom was performed, ***: P≤0.0001, NS: not significant. Coinjection of RNA and morpholino at one cell stage led to partial rescue. The same results were obtained when RNAs were injected at the one-cell stage and the sMOsrsf5a at the 4-cells stage in comparison to embryos injected at four cells stage with the sMOsrsf5a alone.

Acknowledgments

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