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Fig. 7
ISD attenuates Adriamycin induced death and glomerular phenotype in zebrafish: (A) Adriamycin (30.3 mg/liter) was added to zebrafish embryos (8hpf) to induce mortality and glomerular phenotype. The Kaplan-Meier survival curve shows a significant reduction in Adriamycin induced mortality in ISD treated fish (p < 0.05). (B) Adriamycin induced phenotype is categorized into normal, moderate and severe pericardial edema. Quantitative analysis suggests that ISD reduced moderate and severe phenotypes by more than 50%. (C) Histological analysis further revealed a significant reduction in the Adriamycin induced pronephric tubular dilation (a hallmark of renal injury, shown by arrow) and glomerular architecture damage (marked as G).