ZFIN Image: Farwell et al., 2017, Fig. 3

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Fig. 3

Tmem184a MOs-mediated knockdown results in aberrant vascular regeneration. Representative fluorescence microscopy images of fixed 4 dpa Tg(Fli1:EGFP) whole and cryosectioned fins. Each MO was injected into regenerating tissue of fin rays 1–6 at 3 dpa, and the other side was left uninjected. (A) There is no obvious difference in vascular phenotype between the side of a fin injected with Con MO vs. the uninjected side. Con MO n = 12. Injection with ATG MO results in two predominant phenotypes: impaired vascular outgrowth (top) and tangled regenerating vasculature (bottom). ATG MO n = 18. Scale bars = 200 μm. (B) 10x zoomed sections of injected fin shown in (A) allow easier discernment of regenerating vessels. (C) Example transverse maximum intensity projections (bottom) and alpha blended 3D projections (top) show the extent of vessel disorganization in an ATG MO-injected fin ray compared to one injected with Con MO. Scale bars = 50 μm.

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Acknowledgments

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