ZFIN Image: Ma et al., 2017, Fig. S7

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Figure Caption

Fig. S7

Supplementary information for line pIDM-E10. (A) Reduction of the grb14 expression in the pIDM-E10 embryos treated with Dox. The treated embryos were sampled at 24 hpf and subjected to WISH. Anti-sense RNA of grb14 was used to perform WISH. Number, type of represented embryos/total embryos in F₂ population. (B) Induction of the anti-sense transcript of grb14 in the pIDM-E10 embryos treated with Dox was analyzed with qRT-PCR using the primer pairs grb14-intron2-F and grb14-intron2-R. (C) RT-PCR analysis showed that, due to abnormal splicing, the grb14 morpholino (grb14-MO) had created an aberrant transcript with the extra 386 nt. WT embryos injected with the standard control morpholino (SC-MO) were used as the control.

Acknowledgments

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