IMAGE

Fig. S10

ID
ZDB-IMAGE-180126-5
Source
Figures for Lickwar et al., 2017
Image
Figure Caption

Fig. S10

Additional characterization of regulatory capacity of hes1 zCNE_44665 in transgenic zebrafish.

A) 7 dpf cross-section of individual transgenic zebrafish lines for hes1 Tg(zCNE_44665:GFP) show consistent GFP expression in a subset of IECs often at the base of nascent folds. B) Minimal gross overlap can be seen between Notch positive cells in the pancreas and the Tg(zCNE_44665:GFP) signal in 7 dpf zebrafish C) The corpusles of Stannius show high Notch positive signaling and little to no GFP expression from Tg(zCNE_44665:GFP). D) Overlap can be seen between Notch positive cells and Tg(zCNE_44665:GFP) signal in the liver. E) and F) Examples of more apical nuclei in 8 week old cross-section in Tg(zCNE_44665:GFP) and Notch positive cells. G) Whole-mount stereoscopic images of 7 dpf Tg(zCNE_44665:GFP)/Tg(-4.5fabp2:DsRed) shows a large proportion of IECs are DsRed+ enterocytes, however a subset of the IECs not expressing DsRed are GFP+ and are marked by white arrows. H) Whole-mount stereoscopic images of 7 dpf Tg(zCNE_44665:GFP)/Tg(neurod1:TagRFP) show lack of overlap between the hes1 Tg(zCNE_44665:GFP) and enteroendocrine cells. Representative GFP+ cells are marked by a yellow arrow and RFP+ cells are marked by a red arrow. Pancreatic islet marked by i.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.