Fig. S7
Trunk vascular patterning in 15 different radial glia-ablated animals after treatment with Mtz from 30 to 154 hpf. 154 hpf Tg(gfap:NTR);Tg(kdrl:EGFP) animals were treated with Mtz from 30 to 154 hpf. Confocal images were taken using a 20× (A–O) or 40× (A′–O′) objective lens. The region inside the dashed line in A is shown in A′, and the other 40× images (B′–O′) also correspond to the area that covers the four posterior ISVs shown in B–O. VTA formation was affected in all of the animals examined, although vessels were occasionally, and variably, observed in positions where VTAs usually form. Left to right in each image: anterior to posterior parts of the trunk. (Scale bar, 100 µm.)