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Fig. S5

ID
ZDB-IMAGE-180125-1
Source
Figures for Shen et al., 2017
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Figure Caption

Fig. S5

Morphological and WISH analysis of MZikk2-/- embryos. (A-H) Bright field image of wild-type and MZikk2-/- embryos obtained from homozygotic parents at blastula and gastrula stages. MZikk2-/- embryos show disorganized blastoderms (A'-C') unlike compact blastoderm of wild-type embryos (A-C). Epiboly is incomplete (D'-H') and cells detach from yolk. F''-H'', due to delayed epiboly constriction starts before epiboly completion resulting in peanut-shaped embryos. All embryos shown in lateral view. (I-T) Wild-type and MZikk2-/- embryos stained by WISH at shield stage. Embryos stained with dorsal markers (flh, chordin in I-L), ventral markers (bmp2b in M-N) , and mesendoderm markers (ntl, papc, and eve1 in O-P). MZikk2-/- embryos show signs of correct dorsal-ventral patterning and mesendoderm differentiation. I, K, M, O, Q, S: lateral view with dorsal side to the right; J,L,N,P,R,T: animal pole view with dorsal side to the right. Scale bar in all panels: 200 μm.

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