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Fig. S4
Apoptotic cell death in the adult zebrafish olfactory epithelium. A. Immunohistochemistry against the apoptotic marker cleaved caspase 3 (casp3, green, left) and the pan-neuronal marker HuC/D (red, centre) on full sections (top panel) and higher power views (bottom panel) of the intact zebrafish OE. Only occasional caspase 3-positive cells (arrowheads) can be detected. Scale bars: 100 μm. B. Immunohistochemistry against the apoptotic marker cleaved caspase 3 (casp3, green, left and the pan-neuronal marker HuC/D (red, centre) on full sections (top panel) and higher power view (bottom panel) of the adult zebrafish OE 4 h after chemical lesion by nasal irrigation with 1% Triton X-100 for 90 s. Induction of caspase 3 activity is restricted to regions of the OE in which OSNs have been successfully damaged, indicated by the loss of HuC/D immunoreactivity (dotted lines). Scale bars: 100 μm.