Fig. 6
Scratch/wound-healing assay on cardiac endothelial cells. Assay was performed on isolated cardiac endothelial cells from adult Tg(kdrl:EGFP) zebrafish ventricles. (a) Fluorescence and brightfield images at 4 and 16?h after scratching. (b) Quantification of the initial cell free area covered by endothelial cell migration in 12?h. Cell free area at 4?h after scratch was considered as 100% (n?=?3, mean?±?SEM). One way ANOVA followed by Bonferroni?s post-hoc test (GraphPad Prism) was performed to evaluate statistical significance of differences. P?<?0.05 was considered statistically significant. *** corresponds to P?<?0.001.