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Fig. S3
The developmental growth is normal under altered expression of miR-145.
Two mesodermal genes, heart and neural crest derivatives expressed transcript 2 (hand2) at 30 hpf and myogenic differentiation 1(myod) at 54 hpf was examined using WISH. The result reveals mesoderm development was not significantly affected by manipulation of miR-145 expression (A). The body length were measured at 96 hpf after control mimic, miR-145 inhibitor, miR-145 mimic injection in Tg(fabp10:EGFP) embryos (B). (Scale bars, 0.50 mm; thirty embryos per experimental group were used and three independent replicates were performed).
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