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Fig. 1
miR-145 is one of miRNAs prediction on zebrafish GrnA that affects embryonic liver growth.
(A) The miRNAs prediction of GrnA. (B) Liver morphology at 4 dpf was analyzed after control mimic, miR-145 mimic, miR-9 mimic, miR-206 mimic, miR-731 mimic and miR-217 mimic injections in Tg(fabp10:EGFP) embryos. The EGFP expression of Tg (fabp10:EGFP) fish showed a smaller liver phenotype in miR-145 mimic-treated fish compared to wild type, control fish and other miRNAs mimic-treated fish. Thirty embryos per experimental group from one clutch were used and three independent replicates were performed. (C) A 3D image of the liver was analyzed using Leica SP5 confocal microscope and Imaris software. The liver size was examined by measuring the volume of EGFP expression. Ten embryos per experimental group were used and three independent replicates were performed. Scale bars, 100 μm; EGFP, enhanced green fluorescent protein; *P < 0.05, t-test.