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Fig. 2
(A,C) Section from untreated fish, stained for cnr1 receptor, shows a strong stain restricted to parietal ganglia only (arrow head), with low levels of expression in enterocytes or other cell types (A-bar = 200 µm; C- bar = 50 µm). (B) Strong expression of cnr1 is observed in epithelial cells, in some mesenchymal cells, and in ganglia (arrow heads) of the muscular layer of intestine wall of VSL#3-treated fish (bar = 200 µm). (D) Note the strong presence of the receptor in a ganglion, as demonstrated by high power magnification of a particular area in the same section (bar = 50 µm). (E) Intestinal cnr1 protein levels by IHC in control and VSL#3-treated fish. Values indicate mean ± SD. ***p < 0.001.