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Fig. 1
(A) Paraphysiological morphology of intestinal wall in untreated control zebrafish (bar = 200 µm). (B) Morphology of intestine of VSL#3-treated zebrafish reveals good integrity of the gut mucosa, without any inflammatory infiltrate or reactive status to probiotics (bar = 200 µm). (C) In section of gut of untreated fish, a high apoptotic rate evidenced by TUNEL is evident among epithelial and mesenchymal cells, with nuclear intense black stain (bar = 200 µm). (D) TUNEL analysis of section of VSL#3-treated intestine revealed that some enterocytes and mesenchymal cells are going into apoptosis, showing their nuclei intensively black stained by the chromogen (arrow heads, bar = 200 µm). Intestinal (E) casp3, (F) baxa and (G) bcl2a mRNA levels by qPCR normalized against act1b and rplp, in control and VSL#3-treated fish. Values indicate mean ± SD. ***p < 0.001 ****p < 0.0001.