Fig. S6

Control, Gpatch3^ATG, Gpatch3^ex1/2 and Gpatch3^ex2/3 MOs (2.0 ng) were microinjected in 1-2 cells zebrafish embryos. The standard negative control consisted of a MO that targets a human beta-globin intron mutation that causes beta-thalassemia. At 72 hours post fecundation (hpf), larvae were analyzed. Dorsal, lateral and ventral dark field micrographs show altered phenotypes including microphthalmia (blue arrowheads) and abnormal development of pectoral fins (green arrowheads) and craniofacial structures including absence of jaw cartilages (red arrowheads). Scale bars indicate 200 μm.