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Fig. 5
Cytotoxic effects of CFI-400945 in vivo. (A) Comparison of the N-terminal fragment, corresponding to the kinase domain, of human PLK4 (Hs) and the zebrafish ortholog Plk4 (zf). The protein sequences are highly conserved (red: identical amino acids; blue: conserved amino acids), including identical bases involved in CFI-400945 binding sites (highlighted in green). (B) Representative phenotype of a normal zebrafish larva (top) and a zebrafish larva treated with 20 ?M of CFI-400945 that developed pericardial and pronephric duct edema (arrows). (C) Data on the table corresponds to treatment of zebrafish larvae with different concentrations of CFI-400945 at 72 hr