ZFIN Image: Bao et al., 2017, Fig. S2

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Fig. S2

GSK-J1 impaired zebrafish hair cell regeneration. (A–F) 5 dpf larvae were treated with 400 μM neomycin for 1 h followed by GSK-J1 exposure for 24 or 48 h in the presence of BrdU. GSK-J1 significantly reduced the numbers of myosinVI-positive (green) hair cells and BrdU-positive (red) replicating cells. Scale bars = 10 μm. (G,H) Quantification of myosinVI-positive and BrdU-positive cells per neuromast (NM) in DMSO-treated control larvae (Con), 15 μM GSK-J2-treated control larvae, and 15 μM GSK-J1-treated larvae at 24 or 48 h following neomycin damage. In the 24-h group, n = 28 neuromasts of DMSO-treated control larvae (14 larvae), n = 30 neuromasts of 15 μM GSK-J2-treated control larvae (15 larvae), and n = 20 neuromasts of 15 μM GSK-J1-treated larvae (10 larvae); in the 48-h group, n = 18 neuromasts of DMSO-treated control larvae (9 larvae), n = 22 neuromasts of GSK-J2-treated control larvae (11 larvae), and n = 18 neuromasts of 15 μM GSK-J1-treated larvae (9 larvae). ***p < 0.0001. Bars are mean ± sem.

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