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Fig. 3
Stages of CDr10b staining. (a,a') CDr10b taken up at 3 dpf stays in the intestinal lumen and can be used to visualize the timing of cloaca opening. Arrow in a, closed cloaca. (b) CDr10b treatment at 4 dpf results in luminal staining with excretion through the cloaca (arrowheads in a' and b); (c) CDr10b treatment at 5 dpf leads to uptake of the dye specifically by mid-intestinal epithelial cells; (d) The CDr10b signal in the mid-intestine is stable 4 days post treatment (t = 96). dpf, days post fertilization; t, time post treatment.
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