Fig. 2
Dosage and duration of CDr10b treatment. Larvae were treated at 5 dpf with 0.01% DMSO or CDr10b at the doses and duration indicated in the panels, and photographed 24 h post treatment (6 dpf). (a) Brightfield image of a 5 dpf control (DMSO-treated) larva. (a') Same specimen photographed using a red filter set. Only background fluorescence is detectable; (b–e) Increasing incubation times with constant dosage (100 nM CDr10b); (f–i) Increasing dosage of CDr10b with constant incubation time (45 min). All images were captured using the same exposure settings. Red box: Optimal conditions for CDr10b staining. For survival rates see Table S1. ', minutes; hrs, hours; t, time post treatment.