IMAGE

Fig. 4

ID
ZDB-IMAGE-171227-4
Source
Figures for Shao et al., 2017
Image
Figure Caption

Fig. 4

Vrtn overexpression produces dorsalisation and suppresses BMP signalling. (A) Dorsalised embryos at 12 and 24 hpf following Vrtn overexpression. (B) Statistical analysis of Vrtn-dorsalised embryos at 24 hpf. Numbers at the top indicate the total embryos from three independent experiments. (C) Early effects of Vrtn overexpression. The expression patterns of dha/boz and gsc remain unchanged at the sphere stage, and gata2a expression at 70% epiboly and P-Smad1/5/8 level at 60% epiboly are suppressed. The marginal transcription of bmp2b is inhibited at 50% epiboly, and scattered I-YSL expression of bmp2b is present at 70% epibloy. (D-G) Analysis by qRT-PCR of the expression level of dha/boz, gsc, gata2a and bmp2b in Vrtn-overexpressing embryos. The relative expression level in uninjected embryos is normalised as 1; data are mean values±s.d. from four independent experiments. (H) Statistical analysis of the synergistic effect between VAb@2cell and Vrtn overexpression. Numbers at the top indicate the total number of embryos from two independent experiments. Scale bars: 250 µm.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development