ZFIN Image: Lu et al., 2015, Fig. 1

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Figure Caption

Fig. 1

Generation of miR-142a mutants by TALENs. (a) Schematic diagram showed the TALEN target sites at the miR-142a locus. (b and c) Indel mutations induced by TALENs showed a 13-base-pair deletion in the miR-142a locus. Green arrow shows the initiation of the mutation. (d) Predicted RNA secondary structure of wild-type and mutated pri-miR-142a by TALENs using the RNA Structure Software (version 4.6, Rochester, NY, USA). (e) Expression of miR-142a-3p was absent in 142^T−/− embryos at 4 days post-fertilization (dpf) by WISH. Red dashed circles mark the expression of miR-142a-3p in the thymus and red arrowheads mark the expression of miR-142a-3p in the CHT. (f) qPCR demonstrated that the expression of miR-142a-3p and miR-142a-5p was nearly undetectable in 142^T−/− embryos at 4 dpf. The expression of miR-142a-3p and miR-142a-5p was normalized to U6 (mean±s.d., n=3, **P<0.01).

Acknowledgments

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