ZFIN Image: Zhao et al., 2015, Fig. 2

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Figure Caption

Fig. 2

Dorsal, lateral and anterior projections of a 33 hpf Tg(enpep:rtTA; P_Tight:CD2V) zebrafish embryo expressing CD2V sensor in kidney tubules, before and after being treated with 3 mM EGTA, 100 ?M BAPTA-AM and 10 ?M ionomycin for 2 hours.

(a,c) False color fluorescence intensity projections before and after the treatment, with CFP in blue (405-blue channel) and Venus in green (488-green channel). (b,d) CFP lifetime varies along the kidney tubule. After the treatment, CFP lifetime increased, on average, by 0.1?ns over the entire kidney tubule. (e) Average CFP lifetimes of 6 segments of kidney tubules divided equally, before and after the Ca²⁺ treatment. Despite significant variations in average CFP lifetimes between segments, CFP lifetimes in all segments increased after the treatment. Scale bar, 500??m.

Acknowledgments

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