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Fig. 4 S1
Overexpression of atoh1a induces neuromast size through activation of Notch signaling.
(A) Heat-shock strategy to activate atoh1a in the lateral line. (B, C, D, E) her4 expression in the primordium. RT-room temperature. (C) The expression of her4 is even more highly elevated in hs:atoh1a primordia than in NICD primordia (D). (B'–E'') Expression of the atoh1a target deltad in the lateral line. (C') deltad expression is upregulated and expanded in the hs:atoh1a primordia, completely absent in NICD primordia (D') and re-established in some cells in the hs:atoh1a;NICD primordia (E'). (C'') Activation of atoh1a upregulates s100t (hair cell marker) expression in 48 hpf WT neuromasts and induces s100t in several cells in NICD neuromasts (E''). (B–E'') Scale bar is 25 μm. (F) atoh1a overexpression induces L3 neuromast size by itself (light green). Error bars represent standard error from one independent experiment (p<0.05=*, p<0.01=**, p<0.001=*** Student's t test).