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Fig. 1
Pyriproxyfen is toxic at high doses.
(A) Percentage of survival of embryos treated from 2?4 cell stage with various pyriproxyfen concentrations ranging from 0,005??g/ml to 1??g/ml. The latest dose appears to be lethal with 100% of death after 5 days of development. Lower doses of pyriproxyfen (0,005; 0,01; 0,1??g/ml) do not induce a severe decrease in the survival rate (N?=?2 batches, n?=?20 embryos per condition). (B) Higher doses of pyriproxyfen (10 and 100??g/ml) are lethal as early as 2 dpf (N?=?2, n?=?20). (C) Representation of measurements performed on 7 dpf larvae to assess gross morphology effects. (D,E) Eye diameter and body length at 7 dpf of pyriproxyfen-treated embryos are not significantly different from vehicle (EtOH)- treated embryos (p?>?0.05) (n?=?10 per condition).