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Fig. 4
wnt1 in the midbrain is GSK-3 dependent, but is not regulated by β-catenin. Expression of wnt1 (A–H) and lef1 (I–P) was visualised by in situ hybridization in 24 hpf embryos treated with DMSO (A, E, I, M), SU5402 at 10µM (B, G, J, O), BIO at 4µM (C, K), SU5402 and BIO together (D, L), when over-expressing bcat (F, N), when over-expressing bcat and treated with SU5402 (H, P). Isthmus (i). Scale bars: 100µm.
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