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Fig. 5 S1
Inhibiting Ptger2a after gastrulation induces an expansion of the distal early and a restriction of the distal late segment.
(A) Embryos were treated with 0.3% DMSO, 3 μM AH6809, or 3 μM PF04418948 from the 12 ss to the 24 hpf, then fixed and stained for the DE (slc12a1) and DL (slc12a3) using WISH at the 24 hpf stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. The (B) DE and (C) DL domains were measured in microns (n = 5 for each control and experimental group). Data are represented as ± SD, ANOVA used to compare samples, *p<0.01, where n.s. indicates not significant.
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