Fig. 2 S3
Embryos dimensions remain largely unchanged by dmPGE2 or indomethacin treatment.
(A) The pronephric domain (starting at the beginning somite five to the end of somite 18) was measured in microns for embryos treated with 1% DMSO, 100 μM dmPGE2, or 30 μM indomethacin from 4 hpf to 24 hpf (n = 5). (B) The body axis domain at the 24 hpf stage was measured for embryos exposed to 1% DMSO, 100 μM dmPGE2, and 30 μM indomethacin. (C) An image of a 24 hpf embryo stained for slc12a1 and smyhc1, where black bars label the pronephric domain and the body axis domain. Red scale bar, 70 µm. Data are represented as ± SD, ANOVA used to compare samples, where n.s. indicates not significant.