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Fig. S2
Zebrafish Soat2 catalyzes the esterification of cholesterol.
After 2- and 5-hour incubations with 150 μM oleic acids, 10 μg/mL cholesterol and 10 μg/mL NDB-cholesterol, the intracellular accumulation of CEs was observed in the HEK293 cells transiently overexpressing DsRed, zebrafish Soat1-DsRed and zebrafish Soat2-DsRed. The progress of CE accumulation could be seen in cells with zebrafish Soat2 overexpression.
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