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Fig. 8
Delayed yolk consumption by yolk injection of PPPA.
After the injection of PPPA, AVA or DMSO into the yolk at 3 hpf, embryos with grossly normal appearance at 24 hpf were subjected to yolk area measurement to estimate the yolk consumption. Yolk injection of PPPA resulted in significant larger yolk size at 72 and 84 hpf when compared to the DMSO group suggesting a delayed consumption of yolk content. *: P < 0.05, PPPA vs. DMSO; ***: P < 0.001, PPPA vs. DMSO; #: P < 0.05, PPPA vs. AVA
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